Technologist in Molecular Biology: 6 Practice Tests-700+ Q&A
Pass Technologist in Molecular Biology Exam with Confidence: 6 Practice Test with Explanations Covering all Key Topics
Technologist in Molecular Biology: 6 Practice Tests-700+ Q&A free download
Pass Technologist in Molecular Biology Exam with Confidence: 6 Practice Test with Explanations Covering all Key Topics
This course is designed for individuals preparing for the Technologist in Molecular Biology (MB) certification or equivalent industry-level examinations that assess advanced knowledge in molecular biology practices and applications. The course includes 6 full-length practice exams reflecting real-world questions commonly encountered in professional molecular biology roles and standardized exams.
The content of these practice tests is carefully aligned with the core competencies and subject domains expected from a molecular biology technologist, with an emphasis on both theoretical understanding and practical laboratory application. Whether you're a student, a medical technologist transitioning to molecular diagnostics, or a lab professional aiming to validate your readiness, these tests will offer an in-depth evaluation and boost your confidence.
Each question comes with clear explanations, allowing you to reinforce learning, identify weak areas, and gain insights into test patterns. This course is not only a mock test bank—it is a guided tool for revision, review, and real-time test simulation.
Syllabus Covered:
I. MOLECULAR SCIENCE (20–25%)
Nucleic Acid Chemistry: Structures of sugars, bases, DNA/RNA, histones, and mutation types (e.g., point, frameshift).
Basic Molecular Theory: DNA replication, RNA transcription, splicing, translation, chromosome and extrachromosomal structure, and protein folding.
Biochemical Reagents: Detailed understanding of polymerases, nucleases, reverse transcriptase, ligase, primer/probe design.
Genetics: Principles of human and microbial genetics including gene inheritance, mapping, and bacterial mechanisms.
II. MOLECULAR TECHNIQUES (30–35%)
Nucleic Acid Isolation: Techniques for automated and manual extraction.
DNA/RNA Manipulation: Labeling, RFLP, and methylation analysis.
Separation and Detection: Gel/capillary electrophoresis, hybridization methods, probe design (TaqMan, FRET, Molecular Beacons).
Amplification Techniques: PCR types (real-time, nested, multiplex, RT-PCR, digital), and alternatives (NASBA, TMA, LAMP, hybrid capture).
Sequencing: Sanger and Next-Gen Sequencing, RNA-seq, bioinformatics fundamentals.
Other Techniques: Epigenetics, melt-curve analysis, microarrays, MALDI-TOF.
III. LABORATORY OPERATIONS (15–20%)
Contamination Control: Identification, prevention, and elimination.
Quality Assurance: Specimen handling, reagent protocols, internal controls, instrument maintenance, result validation.
Regulatory Compliance: Familiarity with ASR, RUO, IVD, LDP categories, and guidelines from CLIA, CAP, FDA, CMS, TJC, CLSI.
Personnel: Training and competency evaluation.
Safety: Biosafety and chemical hazard protocols.
IV. APPLICATIONS OF MOLECULAR TESTING (30–35%)
Infectious Diseases: Detection, quantification, genotyping of pathogens (e.g., MRSA, HIV, HCV).
Oncology: Molecular diagnostics in hematologic and solid tumors, and hereditary cancer syndromes.
Genetics: Disorders including hemoglobinopathies, coagulopathies, trinucleotide disorders, single-gene mutations, HLA typing.
Other Applications: Forensic testing, transplant engraftment monitoring, and pharmacogenomics (e.g., HER2, CYP450 variants).
